Forschung - Publikationen
Publikationen (ab 2005)
2012
International Patent WO2012/013710 / PCT/EP2011/062921: "Regulation of inducible promoters"; Feb. 2012. Lonza AG, M.Wenzel, J. Altenbuchner, C. Kiziak
2011
K. Morabbi Heravi, M. Wenzel, J. Altenbuchner. Regulation of mtl operon promoter of Bacillus subtilis: Requirements of its use in expression vectors. Microbial Cell Factories 2011, 10:83.
M. Wenzel, A. Müller, M. Siemann-Herzberg, and J. Altenbuchner. Self-inducible Bacillus subtilis expression system enables the reliable and inexpensive protein production by high-cell-density fermentation. Appl Environ Microbiol. 77(18):6419-6425, 2011.
N. Graf, J. Altenbuchner. Development of a method for markerless gene deletion in Pseudomonas putida. Appl Environ Microbiol. 77(15):5549-5552, 2011.
L. Warth, I. Haug, J. Altenbuchner. Characterization of the tyrosine recombinase MrpA encoded by the Streptomyces coelicolor A3(2) plasmid SCP2*. Archives of Microbiology 193(3):187-200, 2011.
H. Beuttler, J. Hoffmann, M. Jeske, B. Hauer, R. D. Schmid, J. Altenbuchner, and V. B. Urlacher. Biosynthesis of zeaxanthin in recombinant Pseudomonas putida. Applied Microbiology and Biotechnology 89:1137-1147, 2011.
2010
H. Motejadded, B. Kranz, S. Berensmeier, M. Franzreb, and J. Altenbuchner. Expression, one-step purification, and immobilization of HaloTag™ fusion proteins on chloroalkane-functionalized magnetic beads. Applied Biochemistry and Biotechnology 162:2098-2110, 2010.
T. Q. Sun and J. Altenbuchner. Characterization of a mannose utilization system in Bacillus subtilis. Journal of Bacteriology 192:2128-2139, 2010.
M. Jeske and J. Altenbuchner. The Escherichia coli rhamnose promoter rhaPBAD is in Pseudomonas putida KT2440 independent of Crp-cAMP activation. Applied Microbiology and Biotechnology 85:1923-1933, 2010.
International Patent WO/2011/018377 / PCT/EP2010/061195: "Fermentation process"; Sept. 2010. Lonza AG, M.Wenzel, J. Altenbuchner, C. Kiziak, M. Siemann-Herzberg
2009
H. Lößner, S. Leschner, A. Endmann, K. Westphal, K. Wolf, K. Kochrübe, T. Miloud, J. Altenbuchner, and S. Weiss. Drug-inducible remote control of gene expression by probiotic Escherichia coli Nissle 1917 in intestine, tumor and gall bladder of mice. Microbes and Infection 11:1097-1105, 2009.
O. Sosedov, K. Matzer, S. Burger, C. Kiziak, S. Baum, J. Altenbuchner, A. Chmura, F. van Rantwijk, and A. Stolz. Construction of recombinant Escherichia coli catalysts which simultaneously express an (S)-oxynitrilase and different nitrilase variants for the synthesis of (S)-mandelic acid and (S)-mandelic amide from benzaldehyde and cyanide. Advanced Synthesis & Catalysis 35:1531-1538, 2009.
H. Motejadded and J. Altenbuchner. Construction of a dual-tag system for gene expression, protein affinity purification and fusion protein processing. Biotechnology Letters3:543-549, 2009.
C. Tolzer, S. Pal, H. Watzlawick, J. Altenbuchner, and K. Niefind. Crystallization and preliminary crystallographic analysis of cgHle, a homoserine acetyltransferase homologue, from Corynebacterium glutamicum. Acta Crystallographica Section F-Structural Biology and Crystallization Communications 65:34-38, 2009.
H. Watzlawick and R. Mattes. Gene cloning, protein characterization, and alteration of product selectivity for the trehalulose hydrolase and trehalulose synthase from "Pseudomonas mesoacidophila" MX-45. Applied and Environmental Microbiology 75:7026-7036, 2009.
G. Placier, H. Watzlawick, C. Rabiller, and R. Mattes. Evolved beta-galactosidases from Geobacillus stearothermophilus with improved transgalactosylation yield for galacto-oligosaccharide production. Applied and Environmental Microbiology75:6312-6321, 2009.
S. Ravaud, X. Robert, H. Watzlawick, R. Haser, R. Mattes, and N. Aghajari. Structural determinants of product specificity of sucrose isomerases. Febs Letters583:1964-1968, 2009.
2008
S. Rustler, H. Motejadded, J. Altenbuchner, and A. Stolz. Simultaneous expression of an arylacetonitrilase from Pseudomonas fluorescens and a (S)-oxynitrilase from Manihot esculenta in Pichia pastoris for the synthesis of (S)-mandelic acid. Applied Microbiology and Biotechnology 80:87-97, 2008.
R. Dürr, A. Neumann, O. Vielhauer, J. Altenbuchner, S. G. Burton, D. A. Cowan, and C. Syldatk. Genes responsible for hydantoin degradation of a halophilic Ochrobactrum sp G21 and Delftia sp 124 - New insight into relation of D-hydantoinases and dihydropyrimidinases. Journal of Molecular Catalysis B-Enzymatic 52-3:2-12, 2008.
A. Wegerer, T. Q. Sun, and J. Altenbuchner. Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combination. BMC Biotechnology 8, 2008.
A. Völker, A. Kirschner, U. T. Bornscheuer, and J. Altenbuchner. Functional expression, purification, and characterization of the recombinant Baeyer-Villiger monooxygenase MekA from Pseudomonas veronii MEK700. Applied microbiology and biotechnology 77:1251-1260, 2008.
S. Ravaud, X. Robert, H. Watzlawick, S. Laurent, R. Haser, R. Mattes, and N. Aghajari. Insights into sucrose isomerization from crystal structures of the Pseudomonas mesoacidophila MX-45 sucrose isomerase, MutB. Biocatalysis and Biotransformation 26:111-119, 2008.
2007
A. Kirschner, J. Altenbuchner, and U. T. Bornscheuer. Design of a secondary alcohol degradation pathway from Pseudomonas fluorescens DSM 50106 in an engineered Escherichia coli. Applied Microbiology and Biotechnology 75:1095-1101, 2007.
C. Onaca, M. Kieninger, K. H. Engesser, and J. Altenbuchner. Degradation of alkyl methyl ketones by Pseudomonas veronii MEK700. Journal of Bacteriology 189:3759-3767, 2007.
A. Kirschner, J. Altenbuchner, and U. T. Bornscheuer. Cloning, expression, and characterization of a Baeyer-Villiger monooxygenase from Pseudomonas fluorescens DSM 50106 in E. coli. Applied Microbiology and Biotechnology 73:1065-1072, 2007.
S. Ravaud, X. Robert, H. Watzlawick, R. Haser, R. Mattes, and N. Aghajari. Trehalulose synthase native and carbohydrate complexed structures provide insights into sucrose isomerization. Journal of Biological Chemistry 282:28126-28136, 2007.
H. Motejadded, J. Altenbuchner. Integration of a lipase gene into Bacillus subtilis chromosome: Recombinant strains without antibiotic resistance marker. Iranian Journal of Biotechnology 5: 105-109, 2007.
2006
H. Gröger, O. May, H. Werner, A. Menzel, and J. Altenbuchner. A "Second-Generation process" for the synthesis of L-neopentylglycine: Asymmetric reductive amination using a recombinant whole cell catalyst. Organic Process Research & Development10:666-669, 2006.
A. Keck, D. Conradt, A. Mahler, A. Stolz, R. Mattes, and J. Klein. Identification and functional analysis of the genes for naphthalenesulfonate catabolism by Sphingomonas xenophaga BN6. Microbiology 152:1929-1940, 2006.
M. Foucault, H. Watzlawick, R. Mattes, R. Haser, and P. Gouet. Crystallization and preliminary X-ray diffraction studies of two thermostable alpha-galactosidases from glycoside hydrolase family 36. Acta Crystallographica Section F-Structural Biology and Crystallization Communications 62:100-103, 2006.
S. Ravaud, H. Watzlawick, R. Haser, R. Mattes, and N. Aghajari. Overexpression, purification, crystallization and preliminary diffraction studies of the Protaminobacter rubrum sucrose isomerase SmuA. Acta Crystallographica Section F-Structural Biology and Crystallization Communications 62:74-76, 2006.
2005
T. Stumpp, S. Himbert, and J. Altenbuchner. Cloning of the netropsin resistance genes from Streptomyces flavopersicus NRRL 2820. Journal of Basic Microbiology 45:355-362, 2005.
B. Apichaisataienchote, J. Altenbuchner, and H. Buchenauer. Isolation and identification of Streptomyces fradiae SU-1 from Thailand and protoplast transformation with the chitinase B gene from Nocardiopsis prasina OPC-131. Current Microbiology 51:116-121, 2005.
S. Ravaud, H. Watzlawick, R. Mattes, R. Haser, and N. Aghajari. Towards the three-dimensional structure of a sucrose isomerase from Pseudomonas mesoacidophila MX-45. Biologia60:89-95, 2005.
C. Kiziak, D. Conradt, A. Stolz, R. Mattes, and J. Klein. Nitrilase from Pseudomonas fluorescens EBC191: cloning and heterologous expression of the gene and biochemical characterization of the recombinant enzyme.
Microbiology 151:3639-3648, 2005.
S. Ravaud, H. Watzlawick, R. Haser, R. Mattes, and N. Aghajari. Expression, purification, crystallization and preliminary X-ray crystallographic studies of the trehalulose synthase MutB from Pseudomonas mesoacidophila MX-45. Acta Crystallographica Section F-Structural Biology and Crystallization Communications 61:100-103, 2005.